Enzymatic Basis of Hygromycin B Resistance in Escherichia coli
نویسنده
چکیده
A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The specific modification of hygromycin B is a phosphorylation of the hydroxyl on the 4 position of the cyclitol ring (hyosamine). The presence of the phosphotransferase in E. coli correlates with reduced accumulation of [14C]hygromycin B.
منابع مشابه
Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli.
The aminoglycoside antibiotic hygromycin B was examined in Escherichia coli cells for inhibitory effects on translation and ribosomal-subunit formation. Pulse-chase labeling experiments were performed, which verified lower rates of ribosomal-subunit synthesis in drug-treated cells. Hygromycin B exhibited a concentration-dependent inhibitory effect on viable-cell numbers, growth rate, protein sy...
متن کاملIdentification of Carbapenem Resistance Genes in Escherichia coli Isolated from Blattella germanica by Dot Blot Assay in Hamadan Hospitals, Iran – 2018
Background and Objective: Today, one of the problems of health systems is the presence of cockroaches in hospitals as insects that move freely in and out of the hospitals and are infected with pathogenic bacteria. The aim of this study was to identify carbapenem resistance genes in Escherichia coli isolated from Blattella germanica by dot blot assay in Hamadan hospitals in the west of Iran. Me...
متن کاملStructural basis for hygromycin B inhibition of protein biosynthesis.
Aminoglycosides are one of the most widely used and clinically important classes of antibiotics that target the ribosome. Hygromycin B is an atypical aminoglycoside antibiotic with unique structural and functional properties. Here we describe the structure of the intact Escherichia coli 70S ribosome in complex with hygromycin B. The antibiotic binds to the mRNA decoding center in the small (30S...
متن کاملCharacterization of β-Lactamases from Urinary Isolates of Escherichia coli in Tehran
Knowledge of antimicrobial resistance patterns in E. coli, the predominant pathogen associated with urinary tract infections (UTI) is important as a guide in selecting empirical antimicrobial therapy. Methods: To describe the antimicrobial susceptibility of E. coli associated with UTI in a major university hospital in Tehran (Iran), seventy-six clinical isolates of E. coli were studied for susc...
متن کاملEvaluation of resistance genes in Escherichia coli isolated from toilets in the Islamic Azad University, Mashhad and genome sequencing (tet B, tet A, tet R, OXA 10 and OXA 48) in tetracycline-resistant strains
Aims and Background: E. coli is a normal flora of the human and animal intestine that sometimes causes pathogenesis in the intestine and urinary tract. The aim of this study is to investigate resistance genes in E. coli isolated from toilets in the Islamic Azad University of Mashhad and perform genome sequencing in tetracycline-resistant strains. Methods: This cross-sectional study was perform...
متن کامل